Sr. Scientist/Principal Scientist, Protein Sciences

Xilio Therapeutics is a clinical-stage biotechnology company discovering and developing tumor-activated immuno-oncology (I-O) therapies with the goal of significantly improving outcomes for people living with cancer without the systemic side effects of current I-O treatments. The company is using its proprietary platform to advance a pipeline of novel, tumor-activated clinical and preclinical I-O molecules that are designed to optimize their therapeutic index by localizing anti-tumor activity within the tumor microenvironment, including tumor-activated cytokines and antibodies (including bispecifics) and immune cell engagers (including tumor-activated cell engagers and tumor-activated effector-enhanced cell engagers). Learn more by visiting www.xiliotx.com and follow us on LinkedIn (Xilio Therapeutics, Inc.)

SUMMARY:

We are looking for a Sr. Scientist/Principal Scientist for our Protein Science Team that will be responsible for mammalian expression and purification of therapeutic proteins. The Scientist will have wide-ranging responsibilities and tremendous opportunities within a dynamic, enthusiastic, highly interdisciplinary and collaborative team.

RESPONSIBILITIES:

  • Optimize and perform the mammalian expression and purification of engineered fusion protein therapeutics.
  • Experience with transient expression from HEK and CHO cells. Stable CHO expression at research scale is preferred.
  • Express proteins from and maintain mammalian cell culture at various scales, small-scale high-throughput screening at microgram quantities to milligram quantities.
  • Perform protein purification using gravity and AKTA-based methods.
  • Purify and characterize expression samples using preparative FPLC and analytical HPLC.
  • Perform characterization and quality assessments (e.g. SDS-PAGE, HPLC, endotoxin) of purified proteins.
  • Proactively investigate different technologies, methods, and emerging tools to increase work efficiency
  • Provide protein science expertise and effectively collaborate with multidisciplinary scientists on cross-functional teams
  • Work with CROs, collaborators and partners to transfer samples, data and protocols into, or out of, Xilio, as appropriate.
  • Enter all data into laboratory notebooks and databases routinely, generate weekly summary reports for management review, and create sample reports for release of protein lots.
  • Regularly present data and analyses at project team and department meetings.

 

QUALIFICATIONS:

  • Ph.D. in Biochemistry or a relevant scientific discipline with 4-7+ years of industry experience or BS/MS with 14 years of experience with therapeutic protein expression and purification.
  • Experience in protein expression of multiple molecule types, including antibodies and fusion proteins from mammalian cell lines, including HEK293 and CHO transient and stable expression platforms
  • Strong technical expertise with AKTA-based protein purification with various chromatography techniques, including affinity, ion exchange, SEC, HIC, etc.
  • Experience with common protein characterization methods, including SDS-PAGE, HPLC, CE-SDS, and endotoxin
  • Experience with robotics, high throughput expression and purification platforms and bioanalytical characterization techniques is highly desirable.
  • A track record of innovation and scientific rigor, strong problem solving and troubleshooting skills, and ability to contribute constructively to discussions.
  • Goal oriented, organized, capable of prioritizing and working on multiple projects, and able to execute to timelines that meet company goals.
  • Work well in a cross functional team environment, both independently as well as be willing to help others.
  • Good verbal and written communication skills.

Diversity, Equity and Inclusion

At Xilio Therapeutics, we foster a culture of equity, diversity and inclusion.  We consider all qualified applicants without regard to race, color, sex, gender identity or expression, sexual orientation, age, religion, national origin, ancestry, ethnicity, disability, veteran status, genetic information, or any other characteristic protected under applicable law.  We will make reasonable accommodations, absent undue hardship, for qualified individuals with known disabilities. If you are an individual with a disability who requires accommodation with the hiring process or to perform any essential functions of the position for which you are applying, please reach out to careers@xiliotx.com.

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