Position Summary:
Twist Biosciences is looking for an enthusiastic and scientifically driven Research Associate II to join our Data Storage team. This position will provide the successful individual with an opportunity to explore DNA synthesis using both phosphoramidite chemistry and enzymatic synthesis. This position will support reagent preparation and experimental operation of custom-made DNA synthesizers, FPLCs and HPLC instruments. You will work collaboratively with other team members to facilitate the routine synthesis and purification of chemically modified oligonucleotides and enzyme-bioconjugates using novel chemistries developed in-house.
The candidate should have a passion for working in a fast-paced and highly multidisciplinary environment, be self-motivated, well-organized, and can execute experiments quickly and efficiently. The candidate should also be comfortable presenting their well organized and analyzed data to the multidisciplinary team.
Active collaboration with colleagues across various disciplines, participation in project team meetings, and contribution toward creative problem solving are encouraged.
What You’ll Be Doing
- Electrochemical and enzymatic DNA Synthesis on Twist’s proprietary DNA synthesis platforms
- Following EHS safety protocols and assisting with maintaining a well-organized, clean, and safe lab working environment. This includes ordering and replenishing an inventory of chemical reagents and lab supplies for daily use
- Preparing reagents, buffers and measuring DNA and enzyme concentrations using the NanoDrop spectrophotometer
- Handling phosphoramidites and other DNA synthesis reagents under a nitrogen atmosphere in a glove box to prepare for electrochemical DNA synthesis
- Synthesizing chemically modified short oligonucleotides containing dye labels, quenchers, photolabile modifications, etc. on a small scale
- Synthesize and purify various enzyme bioconjugates using FPLC, SEC and RP-HPLC instruments per SOP
- Accurately summarize, interpret, and review experimental data with adequate computer skills for data acquisition, data management, data analysis and visualization of experimental results
- Timely and thorough documentation of activities in electronic lab notebook, participate in work presentations at group meetings
What You’ll Bring to the Team
- BS. with at least 8 years experience, M.S. with at least 6-8 years experience in Synthetic Organic Chemistry, Biochemistry, Organic electrochemistry, Nucleic acids chemistry or related fields
- Solid problem solving, excellent communication and time management skills
- Experience in Oligonucleotide synthesis is highly desirable
- Hand-on experience with HPLC, microscopy, LCMS or cyclic voltammetry will be a plus.
About Twist Bioscience
At Twist Bioscience, our expertise is accelerating science and innovation by leveraging the power of scale. We have developed a proprietary semiconductor-based synthetic DNA manufacturing process featuring a high throughput silicon platform capable of producing synthetic biology tools, including genes, oligonucleotide pools and variant libraries. Our platform overcomes the current inefficiencies of synthetic DNA production, and enables cost-effective, rapid, high-quality and high throughput synthetic gene production, which in turn, expedites the design, build, test cycle to enable personalized medicines, pharmaceuticals, sustainable chemical production, improved agriculture production, diagnostics, biodetection and data storage. For more information, please visit www.twistbioscience.com. Twist Bioscience is on Twitter. Sign up to follow our Twitter feed @TwistBioscience at https://twitter.com/TwistBioscience.
Twist Bioscience Corporation is an Equal Opportunity Employer. Twist Bioscience Corporation provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, genetic characteristics, or any other category protected by law.
