With the advent of genomic sequencing, we can finally decode and process our genetic makeup. We now have more data than ever before but providers don't have the infrastructure or expertise to make sense of said data. Here at Tempus, we believe the greatest promise for the detection and treatment of cancer lies in the deep understanding of molecular activity for disease initiation, progression, and efficacious treatment based on the discovery of unique biomarkers.
We're on a mission to connect an entire ecosystem and redefine how genomic data is used in clinical settings. We're looking to build a laboratory team focused on developing state of the art techniques for ultra-deep profiling of the tumor immune microenvironment and experimental validation of novel immuno-oncology features and biomarkers. This team will collaborate across science teams as well as product, research, and business development teams to develop the most advanced platform and dataset in immuno-oncology.
What you will do:
Develop a robust pipeline to functionally characterize and experimentally validate novel immunomodulatory pathways in human cancer.
Perform extensive phenotyping through multiple orthogonal methods (flow cytometry, IHC, single cell RNAseq) to understand the role of different immune pathways within the tumor microenvironment.
Establish novel co-culture systems to model the complex interactions between immune cells, antigen-presenting cells, stromal cells, and tumor cells.
Leverage gene editing technology to explore novel therapeutic avenues in immuno-oncology.
Work with cutting-edge immunology and biological modeling teams to facilitate the development of deep learning algorithms and functional assays.
- PhD with 3+ years of postdoctoral experience
- Demonstrated experience in immunophenotyping and functional characterization of immune genes and pathways, including high-dimensional flow cytometric analysis and sorting, expression profiling, and immunohistochemistry.
- Substantial expertise in cell culture with specific experience with primary human T cells including T cell expansion, activation, in vitro killing, and co-culture assays.
- Fundamental understanding of cancer pathophysiology
- Supervisory experience either in an industry or academic setting
An ideal candidate has:
- Experience preparing and analyzing cells from human tissue for profiling, sequencing, and functional assays
- Previous experience with molecular cloning and methods for gene overexpression, knockdown, and editing (e.g., transient transfection, retroviral/lentiviral infection, siRNA, CRISPR-Cas9)
- Rigorous attention to detail and ability to coordinate complex experimental procedures and workflows with a multidisciplinary team