Shape Therapeutics is a biotechnology company developing breakthrough gene therapy technologies to treat the world’s most challenging diseases. ShapeTX gene therapy platform comprises RNAskip™, RNAfix™, and RNAswitch™ payload technologies, next-generation tissue-specific AAVid™ delivery technology, and SquareBio, a solution to scalable gene therapy manufacturing based on industrialization of human stable cell lines. At the core of these technologies reside ShapeTX AI analytic platform, where data drives decisions in building technology today to enable gene therapies of tomorrow. ShapeTX is committed to data-driven scientific advancement, passionate people, and a mission of providing life-long cures to patients. Shape Life!
At ShapeTX, we are a dynamic team of professionals who are dedicated and passionate about making cures a reality. Through diversity of thought, scientific knowledge, professional rigor and focus we are merging cutting-edge science with extensive drug development expertise to unlock cures to many debilitating diseases.
Shape Therapeutics is headquartered in Seattle, Washington with a satellite site in Cambridge, Massachusetts.
ShapeTX is looking for a molecular biologist with strong Next-Generation Sequencing (NGS) experience to join the AAVidTM Program as a Senior Associate Scientist, AAV Engineering. In this position, the successful candidate will join the AAV Engineering team and develop NGS workflows that allow ShapeTX to discover powerful new gene therapy vectors through massively diverse in vivo screening of AAV capsid variants. The ideal candidate will have significant experience performing NGS techniques, including DNA and RNA isolations from various tissue types, NGS library preparation including molecular- and sample-barcoding techniques, sample pooling and quantitation and analyzing sequencing QC metrics. Candidates should have demonstrated experience in troubleshooting and improving NGS methods. To enable these key experimental workflows you will help maintain lab organization through a diligent and detail oriented working style. In addition, scientific creativity, a collaborative spirit and a desire to work in a fast-paced startup environment are essential.
Roles and Responsibilities:
- Contribute to novel AAV capsid discovery through NGS-based profiling of in vivo selected AAV libraries
- Optimize and develop improved methods for sample preparation, NGS library preparation and library QC techniques
- Perform optimized NGS workflows on critical samples generating consistent, accurate and reliable results
- Keep organized and detailed records using an electronic laboratory notebook and execute well-controlled experiments
- Analyze data promptly to clearly depict and communicate results
- Bachelor's or Master's Degree in Molecular Biology, Virology, Genomics or a related discipline
- 4+ years of NGS experience in an academic or industry setting
- Ability to work both independently and collaboratively in a team-science environment
- Ability to think outside the box and tackle challenges with a problem-solving, can-do attitude
Skills and Experience:
- Experience optimizing nucleic acid isolation from tissue samples (especially animal tissues)
- Experience in developing and troubleshooting novel NGS protocols (e.g. DNA/RNA processing, adaptor ligation, RT/PCR techniques, sample barcoding, molecular barcoding, optimizing cleanup steps)
- Experience quantifying RNA and DNA through qPCR, ddPCR and other methods.
- Experience with synthetic biology/plasmid cloning techniques a plus
- Experience with liquid handling automation a plus
- Experience with AAV biology a plus
- Experience with flow cytometry and/or FACS a plus
- Stellar work ethic and a passion for benchwork
- A collaborative, team player and good lab citizen
- Great attention to detail and organization
- Effective and proactive written and verbal communication