What if…you could join an organization that creates, resources and builds life sciences companies that invent break through technologies in order to transform health care and sustainability?
Flagship Labs 75, Inc. (FL75) is a privately held, early stage biotechnology company that is pioneering the development of multi-specific medicines to deliberately and systematically drug complex biology. FL75 is a highly dynamic, entrepreneurial and innovation-driven organization seeking to hire an exceptional computational scientist to join our team.
FL75 was founded in Flagship’s venture creation engine, where companies such as Moderna Therapeutics (NASDAQ: MRNA), Rubius Therapeutics (NASDAQ: RUBY) and Seres Therapeutics (NASDAQ: MCRB) were conceived and created. Since Flagship’s founding in 2000, the firm has originated and fostered the development of more than 100 scientific ventures resulting in over $34 billion in aggregate value, more than 500 issued patents and more than 50 clinical trials for novel therapeutic agents.
Flagship Labs 75, Inc is looking for a highly motivated and enthusiastic Research Scientist to join our Translational Biology team. This individual will be part of the team that is responsible for the design and execution of cell-based assays for testing the activity of biologicals. This is an exciting opportunity to contribute to the development of the next generation of biologics in a highly collegial environment.
- Establishing mammalian stable cell lines expressing biological targets, from plasmid design to bacterial culture, cloning, transformation, sanger sequencing confirmation, DNA isolation, cell transfection and cell culturing
- Characterization of gene expression in mammalian cells using flow cytometry, microscopy, and qPCR
- Design, execution, and troubleshooting of experiments as well as data analysis
- Collaborate with other teams to develop sensitive high-throughput and automated assays for testing of the activity of biologicals
- Design cell assays tailored to biological targets
Desired Experience and Capabilities
- PhD in biology, synthetic biology, biological engineering, or related field and 2+ years of industry experience
- Expert knowledge of genetic engineering tools applicable to mammalian cells, including design of editing RNAs and accompanying verification strategies
- Extensive hands-on laboratory experience with cell line development and cell engineering techniques, including routine propagation of mammalian cells, multiple transfection reagents/methods, and assessments of cell health and viability
- Experience with both basic and advanced recombinant DNA cloning techniques, including restriction cloning, Golden Gate assembly, Gibson assembly, and PCR-based assembly
- Expertise in virus titration methods, copy number determination, integration site analysis, and molecular biology techniques, such as qPCR, ddPCR, and/or flow cytometry
- Experience in the application of genetic engineering tools to primary immune cells and/or mammalian bioproduction hosts, such as CHO or HEK293T cells strongly preferred
- Willingness and ability to share occasional weekend cell culture duties
- Strong organizational, time management, communication, and interpersonal skills
- Ability to develop and mentor a team of researchers (as needed with team’s expansion)
- Comfort in a fast-paced environment and ability to adjust workload based on changing priorities